4JJY
Alix V domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-30 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.976 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 223.770, 223.770, 223.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.557 - 6.503 |
| R-factor | 0.2107 |
| Rwork | 0.203 |
| R-free | 0.28320 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.550 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.560 | 6.730 |
| High resolution limit [Å] | 6.500 | 6.500 |
| Rmerge | 0.061 | 0.748 |
| Number of reflections | 3774 | |
| <I/σ(I)> | 23.3 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.41 | 22.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 0.1 M sodium citrate pH 5, 6-8% PEG 8000, 5-10% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
