4JHP
The crystal structure of the RPGR RCC1-like domain in complex with PDE6D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97939 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.830, 58.780, 92.110 |
| Unit cell angles | 90.00, 123.32, 90.00 |
Refinement procedure
| Resolution | 29.390 - 1.900 |
| R-factor | 0.20074 |
| Rwork | 0.200 |
| R-free | 0.22128 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jhn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.897 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0093) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.390 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.525 | |
| Number of reflections | 47164 | |
| <I/σ(I)> | 3.43 | |
| Completeness [%] | 99.3 | 99 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M sodium chloride, 0.1M HEPES pH 7.5 and 12% (w/v) PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
