4JHI
Crystal Structure of Medicago truncatula Nodulin 13 (MtN13) in complex with N6-benzyladenine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-13 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.04172 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 96.086, 96.086, 113.584 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.900 - 2.600 |
R-factor | 0.1747 |
Rwork | 0.170 |
R-free | 0.22540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rws |
RMSD bond length | 0.015 |
RMSD bond angle | 1.614 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.900 | 46.900 | 2.760 |
High resolution limit [Å] | 2.600 | 7.710 | 2.600 |
Rmerge | 0.128 | 0.047 | 0.684 |
Number of reflections | 10018 | 451 | 1568 |
<I/σ(I)> | 17 | 38.05 | 4.33 |
Completeness [%] | 99.8 | 97.8 | 99.6 |
Redundancy | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 1.7 M SODIUM MALONATE, 200 mM NaCl, 50 mM Tris-HCl, protein was incubated overnight with N6-benzylopurine prior to crystallization, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |