4JHH

Crystal Structure of Medicago truncatula Nodulin 13 (MtN13) in complex with kinetin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.91801
Spacegroup name	P 62 2 2
Unit cell lengths	96.415, 96.415, 113.407
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.220 - 2.200
R-factor	0.1876
Rwork	0.186
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rws
RMSD bond length	0.020
RMSD bond angle	1.725
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.220	48.220	2.330
High resolution limit [Å]	2.200	6.540	2.200
Rmerge	0.094	0.026	0.647
Number of reflections	16292	721	2433
<I/σ(I)>	14.49	42.19	1.96
Completeness [%]	99.0	98.8	94.4
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	292	1.85 M SODIUM MALONATE, 200 mM NaCl, 50 mM Tris-HCl, protein was incubated overnight with kinetin prior to crystallization, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K