4JHG
Crystal Structure of Medicago truncatula Nodulin 13 (MtN13) in complex with trans-zeatin
Replaces: 3RWSExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 96.110, 96.110, 113.244 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.920 - 1.850 |
R-factor | 0.1837 |
Rwork | 0.182 |
R-free | 0.21610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qim |
RMSD bond length | 0.019 |
RMSD bond angle | 1.578 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.920 |
High resolution limit [Å] | 1.850 | 3.980 | 1.850 |
Rmerge | 0.075 | 0.035 | 0.981 |
Number of reflections | 26831 | ||
<I/σ(I)> | 9.6 | 52.6 | 2.1 |
Completeness [%] | 99.9 | 99.4 | 100 |
Redundancy | 10.5 | 9.9 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 1.9 M SODIUM MALONATE, 200 mM NaCl, 50 mM Tris-HCl, protein was incubated overnight with trans-zeatin prior to crystallization, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |