4JGX
The Structure of Sterol Carrier Protein 2 from the Yeast Yarrowia Lipolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-09 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 67.350, 67.350, 117.020 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.190 - 2.200 |
| R-factor | 0.18815 |
| Rwork | 0.187 |
| R-free | 0.21714 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ikt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.277 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.190 | 2.257 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 16152 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 1.4M SODIUM CITRATE, 100MM HEPES, pH 6.5, VAPOR DIFFUSION, temperature 293K |
