4JGE

Crystal structure of red fluorescent gene-engineered variant with improved folding - lanRFP_delS83 (Branchiostoma lanceolatum)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 64 2 2
Unit cell lengths	133.762, 133.762, 156.956
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.760 - 1.940
R-factor	0.1566
Rwork	0.156
R-free	0.19820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jf9
RMSD bond length	0.033
RMSD bond angle	2.332
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.010
High resolution limit [Å]	1.940	4.180	1.940
Rmerge	0.109	0.066	0.425
Number of reflections	60055
<I/σ(I)>	7.9
Completeness [%]	97.2	97.6	89.1
Redundancy	4	3.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	35.6 mg/ml of lanRFP- S83 in 20 mM Tris pH 8.0, 200 mM NaCl, 5 mM EDTA mixed with an equal amount of reservoir solution - 0.2M Na2HPO4 0.1M Tris pH 7.0 20% PEG3350 , VAPOR DIFFUSION, HANGING DROP, temperature 293.0K