4JG8

Structure of RSK2 T493M CTD mutant bound to 2-cyano-N-(1-hydroxy-2-methylpropan-2-yl)-3-(3-(3,4,5-trimethoxyphenyl)-1H-indazol-5-yl)acrylamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-10-21
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	46.350, 46.350, 292.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.778 - 3.100
R-factor	0.2394
Rwork	0.237
R-free	0.29780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qr8
RMSD bond length	0.002
RMSD bond angle	0.452
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.760	3.290
High resolution limit [Å]	3.100	3.100
Number of reflections	6457
Completeness [%]	99.9	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	100 mM HEPES, 50 mM Ammonium Sulfate, 7.5% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K