4JEI
Nonglycosylated Yarrowia lipolytica LIP2 lipase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 116.693, 116.693, 170.272 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.970 - 2.600 |
| R-factor | 0.2251 |
| Rwork | 0.224 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dte |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.730 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.120 | 56.000 |
| Number of reflections | 21708 | |
| <I/σ(I)> | 17.6 | 2.7 |
| Completeness [%] | 96.6 | 99.5 |
| Redundancy | 12 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1M Li2SO4, 2% PEG 8000 (pH 6.5)., VAPOR DIFFUSION, SITTING DROP, temperature 293K |
