4JE8

Crystal structure of a human-like mitochondrial peptide deformylase in complex with Met-Ala-Ser

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Collection date	2009-04-09
Spacegroup name	P 21 21 21
Unit cell lengths	51.550, 74.710, 109.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.680 - 2.400
R-factor	0.17761
Rwork	0.174
R-free	0.25096
Starting model (for MR)	1zxz
RMSD bond length	0.030
RMSD bond angle	2.667
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC (5.2.0019)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.400	2.390
Number of reflections	16326
<I/σ(I)>	11.68	3.03
Completeness [%]	99.5	97.2
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	5-15% PEG 5000 MME, 100mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K