4JE7

Crystal structure of a human-like mitochondrial peptide deformylase in complex with actinonin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Collection date	2007-03-14
Spacegroup name	P 21 21 21
Unit cell lengths	51.840, 74.320, 109.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.880 - 2.100
R-factor	0.18216
Rwork	0.179
R-free	0.23061
Starting model (for MR)	1zxz
RMSD bond length	0.020
RMSD bond angle	1.927
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC (5.2.0019)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.000	2.000
Number of reflections	28781
<I/σ(I)>	18.95	2.87
Completeness [%]	97.5	93.5
Redundancy	4.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	5-15% PEG 5000 MME, 100mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K