4JE5
Crystal structure of the aromatic aminotransferase Aro8, a putative alpha-aminoadipate aminotransferase in Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 227.442, 159.831, 70.642 |
| Unit cell angles | 90.00, 106.03, 90.00 |
Refinement procedure
| Resolution | 34.250 - 1.909 |
| R-factor | 0.1838 |
| Rwork | 0.182 |
| R-free | 0.21750 |
| Structure solution method | SAD, MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.370 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 1.980 |
| High resolution limit [Å] | 1.909 | 4.110 | 1.909 |
| Rmerge | 0.065 | 0.032 | 0.461 |
| Number of reflections | 181016 | ||
| <I/σ(I)> | 12.7 | ||
| Completeness [%] | 96.8 | 98.9 | 78.4 |
| Redundancy | 4.3 | 4.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | HEPES, Sodium Citrate, pH 7.4, vapor diffusion, hanging drop, temperature 298K |
