4JCJ
Crystal structure of Isl1 LIM domains with Ldb1 LIM-interaction domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2816 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 57.310, 56.639, 179.342 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.290 - 3.000 |
| R-factor | 0.2556 |
| Rwork | 0.254 |
| R-free | 0.29870 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.304 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.160 | 0.660 |
| Number of reflections | 12362 | |
| <I/σ(I)> | 8.5 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 298 | 20% (w/v) PEG 8000, 0.2 M magnesium chloride, 0.1 M Tris, 0.3 mM zinc sulfate, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
