4JCE
L54F Variant of JC Polyomavirus Major Capsid Protein VP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-15 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.430, 95.710, 128.800 |
| Unit cell angles | 90.00, 110.27, 90.00 |
Refinement procedure
| Resolution | 44.530 - 1.900 |
| R-factor | 0.15093 |
| Rwork | 0.149 |
| R-free | 0.18487 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nxg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.415 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 132607 | |
| <I/σ(I)> | 15.14 | 2.55 |
| Completeness [%] | 99.3 | 96.9 |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES, 0.2 M KSCN, 12% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
