4JBR

tRNA-guanine transglycosylase Y330C mutant as covalently linked dimer in space group P6(5)22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.91841
Spacegroup name	P 65 2 2
Unit cell lengths	127.552, 127.552, 116.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.111 - 2.920
R-factor	0.2053
Rwork	0.203
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pud
RMSD bond length	0.003
RMSD bond angle	0.665
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.970
High resolution limit [Å]	2.920	2.920
Number of reflections	12637
<I/σ(I)>		6.06
Completeness [%]	100.0	100
Redundancy	15	16.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	MgAc 0.2M, PEG3350 20%; 2% DMSO and 10% glycerol in cryoprotection buffer, VAPOR DIFFUSION, SITTING DROP, temperature 293K