4JAM
Crystal structure of broadly neutralizing anti-hiv-1 antibody ch103
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.027, 146.375, 66.322 |
| Unit cell angles | 90.00, 97.71, 90.00 |
Refinement procedure
| Resolution | 38.185 - 1.650 |
| R-factor | 0.1703 |
| Rwork | 0.169 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3se9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.160 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.530 | |
| Number of reflections | 95840 | |
| <I/σ(I)> | 30 | 2.6 |
| Completeness [%] | 98.4 | 90.9 |
| Redundancy | 3.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 170 mM ammonium sulfate, 25.5 % PEG 4000, 15% Glycerol, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
