4JAM
Crystal structure of broadly neutralizing anti-hiv-1 antibody ch103
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.027, 146.375, 66.322 |
Unit cell angles | 90.00, 97.71, 90.00 |
Refinement procedure
Resolution | 38.185 - 1.650 |
R-factor | 0.1703 |
Rwork | 0.169 |
R-free | 0.19700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3se9 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.160 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.530 | |
Number of reflections | 95840 | |
<I/σ(I)> | 30 | 2.6 |
Completeness [%] | 98.4 | 90.9 |
Redundancy | 3.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 170 mM ammonium sulfate, 25.5 % PEG 4000, 15% Glycerol, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |