4JA0
Crystal structure of the invertebrate bi-functional purine biosynthesis enzyme PAICS at 2.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 138.729, 219.726, 60.645 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.711 - 2.800 |
| R-factor | 0.2026 |
| Rwork | 0.199 |
| R-free | 0.26260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h31 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.245 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.072 | |
| Number of reflections | 46715 | |
| <I/σ(I)> | 21.4 | 2.6 |
| Completeness [%] | 1.0 | 97.4 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 50mM Tris, 2M Ammonium sulfate, 1% PEG400, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
