4J9U

Crystal Structure of the TrkH/TrkA potassium transport complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.2515
Spacegroup name	P 1 21 1
Unit cell lengths	133.725, 146.627, 163.672
Unit cell angles	90.00, 99.32, 90.00

Refinement procedure

Resolution	49.791 - 3.800
R-factor	0.2342
Rwork	0.232
R-free	0.28010
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.242
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.870
High resolution limit [Å]	3.800	10.290	3.800
Rmerge	0.144	0.097	0.814
Number of reflections	61373
<I/σ(I)>	8.6
Completeness [%]	99.7	98.7	98.7
Redundancy	7.2	7.4	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.75	298	37.5% PEG 400, 400 mM ammonium sulfate, 100 mM HEPES, pH 6.75, microbatch, temperature 298K