4J8Z
Crystal Structure of the Human SPOP BTB Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-01 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.91730 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 111.390, 71.580, 62.220 |
Unit cell angles | 90.00, 117.21, 90.00 |
Refinement procedure
Resolution | 58.020 - 2.420 |
R-factor | 0.2091 |
Rwork | 0.207 |
R-free | 0.25490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.583 |
Data reduction software | XDS |
Data scaling software | SCALA (0.1.27) |
Phasing software | PHASER (2.5.2) |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 58.020 |
High resolution limit [Å] | 2.420 |
Rmerge | 0.044 |
Number of reflections | 16513 |
<I/σ(I)> | 17.2 |
Completeness [%] | 98.9 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 32% PEG3350, 17% isopropanol, 0.1M tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |