4J7Q
Crystal structure of Saccharomyces cerevisiae Sfh3 complexed with phosphatidylinositol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.889, 144.149, 55.656 |
| Unit cell angles | 90.00, 111.28, 90.00 |
Refinement procedure
| Resolution | 34.400 - 1.550 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.23900 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| <I/σ(I)> | 32.4 | 4.3 |
| Completeness [%] | 99.1 | 98.8 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.1M MES, 20% PEG 8000, 0.2M ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
