4J7E
The 1.63A crystal structure of humanized Xenopus MDM2 with a nutlin fragment, RO5524529
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2012-09-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.290, 68.612, 67.349 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.310 - 1.630 |
| R-factor | 0.223 |
| Rwork | 0.222 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.540 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.061 | 0.998 |
| Number of reflections | 16986 | |
| <I/σ(I)> | 7.9 | 0.9 |
| Completeness [%] | 98.5 | 98.5 |
| Redundancy | 6.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | 40-50% SATURATED AMMONIUM SULFATE, 0.1m MES, PH 6.5, 5% PEG200, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
