4J5V
Crystal structure of p19 in complex with double-helical RNA 19mer p(CAG)3C(CCG)3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9724 |
Spacegroup name | H 3 2 |
Unit cell lengths | 90.219, 90.219, 148.039 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.150 |
R-factor | 0.17369 |
Rwork | 0.172 |
R-free | 0.21401 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 2.114 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.190 |
High resolution limit [Å] | 2.150 | 5.820 | 2.150 |
Rmerge | 0.100 | 0.056 | 0.134 |
Number of reflections | 12580 | ||
<I/σ(I)> | 19.5 | ||
Completeness [%] | 98.2 | 98.9 | 95.9 |
Redundancy | 3.1 | 4.2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 1.7 mM ammonium sulfate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |