4J5O
Room temperature crystal structure of a RNA binding motif protein 39 (Rbm39) from Mus musculus at 1.11 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 296 |
Detector technology | PIXEL |
Collection date | 2012-05-30 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.617, 52.829, 85.652 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.551 - 1.110 |
R-factor | 0.1134 |
Rwork | 0.113 |
R-free | 0.12890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s6e |
RMSD bond length | 0.012 |
RMSD bond angle | 1.491 |
Data reduction software | XDS |
Data scaling software | XSCALE (March 15, 2012) |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.551 | 28.550 | 1.140 |
High resolution limit [Å] | 1.110 | 4.970 | 1.110 |
Rmerge | 0.055 | 0.033 | 1.016 |
Number of reflections | 75696 | 917 | 3368 |
<I/σ(I)> | 18.4 | 67.3 | 2.2 |
Completeness [%] | 94.2 | ||
Redundancy | 9.7 | 10.4 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 20.00% polyethylene glycol 6000, 0.1M sodium citrate pH 5.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |