4J4Y
Triple mutant GraVN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 54.160, 76.798, 77.156 |
Unit cell angles | 82.79, 69.51, 69.36 |
Refinement procedure
Resolution | 28.810 - 2.450 |
R-factor | 0.21412 |
Rwork | 0.210 |
R-free | 0.29102 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j4r |
RMSD bond length | 0.012 |
RMSD bond angle | 1.639 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.830 | 2.590 |
High resolution limit [Å] | 2.450 | 2.500 |
Number of reflections | 35932 | |
Completeness [%] | 95.2 | 91.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M Ca(OAc)2, 12%(w/v) PEG3350, 0.04M citric acid, 0.06M Bis-tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |