4J4G
Structure of P51G Cyanovirin-N swapped tetramer in the C2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-11-28 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.754, 46.232, 117.608 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 29.400 - 1.920 |
| R-factor | 0.20763 |
| Rwork | 0.204 |
| R-free | 0.24111 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ezm |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.529 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.109 | 0.392 |
| Number of reflections | 40086 | |
| <I/σ(I)> | 8.4 | 1.9 |
| Completeness [%] | 97.9 | 89.9 |
| Redundancy | 5.68 | 3.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 0.8 M potassium phosphate dibasic, 1.2 M sodium phosphate monobasic, 0.1 M CAPS, pH 10.5, 0.2 M lithium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
