4J3D
Pseudomonas aeruginosa LpxC in complex with a hydroxamate inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.762, 156.878, 49.334 |
| Unit cell angles | 90.00, 102.40, 90.00 |
Refinement procedure
| Resolution | 156.878 - 2.000 |
| R-factor | 0.2047 |
| Rwork | 0.201 |
| R-free | 0.28110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.433 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 156.878 | 2.050 |
| High resolution limit [Å] | 1.830 | 2.000 |
| Number of reflections | 35689 | |
| Completeness [%] | 90.0 | 92.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 13-18% P3350, 15-75mM CaCl2, 4% Isopropanol and 0.1M HEPES pH 7.5 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
