4J30
Structure of the effector - immunity system Tae4 / Tai4 from Salmonella typhimurium, selenomethionine variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 63.380, 63.380, 364.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.800 - 2.300 |
| R-factor | 0.2001 |
| Rwork | 0.198 |
| R-free | 0.23138 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.154 | 0.587 |
| Number of reflections | 36560 | |
| <I/σ(I)> | 24 | 7.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 42 | 43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 200 mM tri-sodium citrate pH 6.0, 30 % (v/v) 2-ethoxyethanol , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
