4J2G
Atg13 HORMA domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2012-05-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.650, 65.670, 85.760 |
| Unit cell angles | 90.00, 101.46, 90.00 |
Refinement procedure
| Resolution | 57.250 - 2.290 |
| R-factor | 0.21689 |
| Rwork | 0.214 |
| R-free | 0.26279 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.052 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 84.050 |
| High resolution limit [Å] | 2.290 |
| Completeness [%] | 99.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.2mM ammonium sulfate, 0.1M MES pH6.5, 20% w/v PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
