4J25
Crystal structure of a Pseudomonas putida prolyl-4-hydroxylase (P4H)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979500 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.330, 62.160, 132.830 |
| Unit cell angles | 91.84, 94.70, 90.08 |
Refinement procedure
| Resolution | 62.127 - 1.970 |
| R-factor | 0.2127 |
| Rwork | 0.212 |
| R-free | 0.25730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g1m |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.049 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (0.1.16) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.130 | 2.020 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.069 | 0.551 |
| Number of reflections | 99718 | |
| <I/σ(I)> | 10.6 | 2.3 |
| Completeness [%] | 97.7 | 96.7 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1M MIB buffer pH 5.0, 25% PEG 1500, 2mM MnCl2, 5mM OGA, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
