4J19

Structure of a novel telomere repeat binding protein bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	110
Detector technology	PIXEL
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	111.433, 116.490, 75.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.155 - 2.900
R-factor	0.1741
Rwork	0.172
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cuf
RMSD bond length	0.005
RMSD bond angle	0.989
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1218))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.155
High resolution limit [Å]	2.900
Number of reflections	11682
Completeness [%]	95.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	24% PEG 3350, 100 mM sodium acetate, 100 mM potassium sulfate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K