4J0X
Structure of Rrp9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.445, 78.557, 80.029 |
| Unit cell angles | 61.77, 86.44, 71.99 |
Refinement procedure
| Resolution | 19.963 - 2.502 |
| R-factor | 0.2199 |
| Rwork | 0.218 |
| R-free | 0.26160 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.934 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.088 | 0.319 |
| Number of reflections | 32300 | |
| <I/σ(I)> | 9 | 1.7 |
| Completeness [%] | 95.8 | 85.1 |
| Redundancy | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100mM HEPES-K, pH7.5, 25% (v/v) ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
