4J0W
Structure of U3-55K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.651, 63.651, 167.425 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.21102 |
| Rwork | 0.210 |
| R-free | 0.23188 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j0x |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.123 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.118 | 0.718 |
| Number of reflections | 38893 | |
| <I/σ(I)> | 32.3 | 4.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.7 | 16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 277 | 100mM HEPES-K, 25% (v/v) ethylene glycol, pH 8.0, VAPOR DIFFUSION, temperature 277K |
