4J0C
tannin acyl hydrolase from Lactobacillus plantarum (native structure)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.458, 62.791, 83.803 |
| Unit cell angles | 70.39, 86.01, 79.41 |
Refinement procedure
| Resolution | 19.677 - 1.650 |
| R-factor | 0.1661 |
| Rwork | 0.165 |
| R-free | 0.19190 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.187 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.740 | |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.077 | 0.550 |
| Number of reflections | 100941 | |
| Completeness [%] | 88.2 | |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 281 | 0.1M tri-sodium citrate pH 5.5, 20% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 281K |
