4IZX
Macrolepiota procera ricin B-like lectin (MPL) in complex with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.800, 57.158, 61.229 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.220 - 1.100 |
| R-factor | 0.1086 |
| Rwork | 0.107 |
| R-free | 0.13210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ion |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.558 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 1.120 |
| High resolution limit [Å] | 1.100 | 2.980 | 1.100 |
| Rmerge | 0.050 | 0.024 | 0.387 |
| Number of reflections | 43310 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 88.2 | 95.5 | 42.9 |
| Redundancy | 7 | 6.4 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M Imidazole, 0.5 M Sodium citrate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
