4IZV
The E41Q/C145A double mutant of the amidase from Nesterenkonia sp. AN1 in complex with acrylamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-14 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.446, 114.932, 65.554 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.720 - 1.650 |
| R-factor | 0.171 |
| Rwork | 0.170 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.278 |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.213 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.389 | |
| Number of reflections | 34247 | |
| <I/σ(I)> | 18 | 1.7 |
| Completeness [%] | 98.9 | 93.4 |
| Redundancy | 6.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 298 | 2.0 M ammonium sulfate, vapor diffusion, sitting drop, temperature 298K |
