4IZM
Crystal structure of GltPh L66C-S300C mutant crosslinked with divalent mercury
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 61 |
| Unit cell lengths | 119.708, 119.708, 333.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 4.500 |
| R-factor | 0.25259 |
| Rwork | 0.250 |
| R-free | 0.29879 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.138 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.600 |
| High resolution limit [Å] | 3.540 | 9.590 | 3.540 |
| Rmerge | 0.097 | 0.034 | |
| Number of reflections | 15950 | ||
| <I/σ(I)> | 2.4 | ||
| Completeness [%] | 99.9 | 98.4 | 100 |
| Redundancy | 6.4 | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 277.15 | PEG 1000, 100mM Li2SO4, 50mM citric acid, 50mM Na2HPO4, hanging drop, temperature 277.15K |
