4IZH
Crystal Structure of the Alpha1 dimer of Thermus thermophilus Transhydrogenase in P6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 6 |
| Unit cell lengths | 162.318, 162.318, 73.883 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 140.610 - 1.800 |
| R-factor | 0.16754 |
| Rwork | 0.167 |
| R-free | 0.18024 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l7d |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.525 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 140.610 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.437 |
| Number of reflections | 97773 | |
| <I/σ(I)> | 11.3 | 1.7 |
| Completeness [%] | 99.5 | 97.4 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 3.5 | 293 | MemStart MemSys kit, condition E2: 0.1M Sodium chloride, 0.1M Na citrate pH 3.5, 0.1M Lithium sulphate, 30% v/v PEG 400, VAPOR DIFFUSION, temperature 293K |
