4IY9
Bmlp3 - C2 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.150, 34.560, 93.140 |
| Unit cell angles | 90.00, 97.99, 90.00 |
Refinement procedure
| Resolution | 38.100 - 2.100 |
| R-factor | 0.2188 |
| Rwork | 0.216 |
| R-free | 0.27150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4efp |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.810 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.164 | 2.184 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 | 0.496 |
| Number of reflections | 26553 | |
| <I/σ(I)> | 15.9 | 2.58 |
| Completeness [%] | 91.3 | 86.3 |
| Redundancy | 3.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2.0 M (NH4)2SO4, 0.1 M NH4I, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
