4IXZ

Native structure of cystathionine gamma lyase (XometC) from xanthomonas oryzae pv. oryzae at pH 9.0

Replaces:  3NMY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-11
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	78.817, 86.308, 223.351
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.250 - 2.070
R-factor	0.184
Rwork	0.181
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e6g
RMSD bond length	0.024
RMSD bond angle	1.972
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	2.070	2.070
Number of reflections	93642
Completeness [%]	99.3	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	25.5% PEG 4000, 15% GLYCEROL, 0.17MM LITHIUM SULFATE, 0.085MM TRIS, PH 9.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K