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4IXM

Crystal structure of Zn(II)-bound YjiA GTPase from E. coli

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL9-2
Synchrotron siteSSRL
BeamlineBL9-2
Temperature [K]100
Detector technologyCCD
Collection date2007-07-21
DetectorMARMOSAIC 325 mm CCD
Wavelength(s)1.1808
Spacegroup nameP 1 21 1
Unit cell lengths56.295, 68.869, 77.829
Unit cell angles90.00, 104.09, 90.00
Refinement procedure
Resolution29.126 - 2.570
R-factor0.2221
Rwork0.220
R-free0.26230
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1nij
RMSD bond length0.002
RMSD bond angle0.560
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwarePHASER
Refinement softwarePHENIX ((phenix.refine: 1.8.1_1168))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.610
High resolution limit [Å]2.5702.570
Number of reflections35529
<I/σ(I)>18.33.3
Completeness [%]97.084.6
Redundancy3.83.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.92981.5 uL of 13.2 mg/mL YjiA in 0.1 M NaCl, 25 mM HEPES, pH 7.6, mixed with 0.3 uL 0.1 M CaCl2 and 1.5 uL 1.55 M ammonium sulfate, 0.1 M , VAPOR DIFFUSION, HANGING DROP, temperature 298K

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