4IXM
Crystal structure of Zn(II)-bound YjiA GTPase from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-21 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.1808 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.295, 68.869, 77.829 |
| Unit cell angles | 90.00, 104.09, 90.00 |
Refinement procedure
| Resolution | 29.126 - 2.570 |
| R-factor | 0.2221 |
| Rwork | 0.220 |
| R-free | 0.26230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nij |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.560 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.610 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Number of reflections | 35529 | |
| <I/σ(I)> | 18.3 | 3.3 |
| Completeness [%] | 97.0 | 84.6 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 298 | 1.5 uL of 13.2 mg/mL YjiA in 0.1 M NaCl, 25 mM HEPES, pH 7.6, mixed with 0.3 uL 0.1 M CaCl2 and 1.5 uL 1.55 M ammonium sulfate, 0.1 M , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
