4IXL
Crystal structure of endo-beta-1,4-xylanase from the alkaliphilic Bacillus sp. SN5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Detector technology | CCD |
| Collection date | 2012-03-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 43 |
| Unit cell lengths | 59.415, 59.415, 153.736 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.812 - 1.490 |
| Rwork | 0.196 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dck |
| Data reduction software | APEX |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.490 |
| Rmerge | 0.027 |
| Number of reflections | 85897 |
| Completeness [%] | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 0.1 M lithium sulfate monohydrate, 0.1 M ADA pH 6.5, 12% w/v PEG4000, 2% v/v 2-Propanol, VAPOR DIFFUSION, SITTING DROP |
