4IWM

Crystal Structure of the Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii (in P21 form)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	173
Detector technology	CCD
Collection date	2013-01-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97921, 0.97938, 0.96414
Spacegroup name	P 1 21 1
Unit cell lengths	95.370, 77.136, 131.737
Unit cell angles	90.00, 105.36, 90.00

Refinement procedure

Resolution	26.901 - 2.700
R-factor	0.1921
Rwork	0.189
R-free	0.25770
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.136
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	30.000
High resolution limit [Å]	2.700	2.700
Rmerge		0.450
Number of reflections	51206
<I/σ(I)>		2.8
Completeness [%]	99.7	99.8
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	0.1M (NH4)2SO4, 0.3M sodium formate, 0.1M sodium acetate, 3% PGA-LM, 20% MPD, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 277K