4IW7
Crystal structure of 8-amino-7-oxononanoate synthase (bioF) from Francisella tularensis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 113.486, 113.486, 158.128 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.770 - 2.250 |
| R-factor | 0.18828 |
| Rwork | 0.186 |
| R-free | 0.23836 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g6w |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.536 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.079 | 0.742 |
| Number of reflections | 18866 | |
| <I/σ(I)> | 29.7 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.9 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.7 M Sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
