4IW0

Crystal structure and mechanism of activation of TBK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	77
Detector technology	CCD
Collection date	2012-06-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.939
Spacegroup name	P 65 2 2
Unit cell lengths	228.910, 228.910, 97.980
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.559 - 4.000
R-factor	0.2401
Rwork	0.237
R-free	0.29110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.518
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	4.000
Number of reflections	13213
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	5% PEG8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K