4IVE

Crystal structure of a polyadenylate-binding protein 3 (PABPC3) from Homo sapiens at 2.30 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979493,0.918401,0.979284
Spacegroup name	P 43
Unit cell lengths	67.395, 67.395, 101.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.128 - 2.300
R-factor	0.2011
Rwork	0.201
R-free	0.21210
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	XSCALE (March 15, 2012)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.128	43.128	2.380
High resolution limit [Å]	2.300	4.930	2.300
Rmerge	0.097	0.053	0.756
Number of reflections	20106	2103	1919
<I/σ(I)>	12.03	25	2.4
Completeness [%]	99.5	99.7	97.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.62	277	26.00% polyethylene glycol 6000, 0.1M MES pH 5.62, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K