4IV1
Crystal structure of recombinant foot-and-mouth-disease virus A22 empty capsid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 294 |
| Detector technology | PIXEL |
| Collection date | 2012-03-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 327.628, 341.374, 363.701 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.980 - 2.100 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gh4 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.244 | |
| Number of reflections | 869280 | |
| <I/σ(I)> | 3.6 | 0.5 |
| Completeness [%] | 74.6 | 25.8 |
| Redundancy | 2.7 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 4 M ammonium acetate, 100 mM bis-Tris Propane, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
