4IUW

Crystal structure of PEPO from Lactobacillus rhamnosis HN001 (DR20)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002+
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2005-03-11
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	56.387, 83.756, 143.828
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.240 - 1.850
R-factor	0.16554
Rwork	0.163
R-free	0.22105
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zuk
RMSD bond length	0.017
RMSD bond angle	1.665
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.240	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.081	0.156
Number of reflections	46976
<I/σ(I)>	15.1	3.9
Completeness [%]	79.6
Redundancy	6.13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	1.0M LITHIUM SULPHATE MONOHYDRATE, 0.1M TRI-SODIUM CITRATE DEHYDRATE, 0.1M AMMONIUM SULPHATE, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K