4IU6
Human Methionine Aminopeptidase in complex with FZ1: Pyridinylquinazolines Selectively Inhibit Human Methionine Aminopeptidase-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-19 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.303, 77.344, 47.821 |
| Unit cell angles | 90.00, 91.49, 90.00 |
Refinement procedure
| Resolution | 47.800 - 1.900 |
| R-factor | 0.175 |
| Rwork | 0.172 |
| R-free | 0.22270 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.432 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.055 | 0.038 | 0.277 |
| Number of reflections | 24986 | ||
| <I/σ(I)> | 17.1 | ||
| Completeness [%] | 91.6 | 93.2 | 59.7 |
| Redundancy | 3 | 3.2 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
