4IT4

Crystal structure of residues 1-211 of CG17282

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	66.098, 90.193, 249.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.650 - 2.500
R-factor	0.1987
Rwork	0.197
R-free	0.23030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Residues 1-120 of CG17282
RMSD bond length	0.008
RMSD bond angle	0.962
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.129	0.095	0.695
Number of reflections	53197
<I/σ(I)>	12.7
Completeness [%]	99.9	99.4	99.7
Redundancy	7.5	7.8	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	8% (v/v) Tacsimate pH 4.5, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K