4IT1
Crystal structure of enolase pfl01_3283 (target efi-502286) from pseudomonas fluorescens pf0-1 with bound magnesium, potassium and tartrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-03 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.321, 147.695, 173.195 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.400 - 2.200 |
| R-factor | 0.19566 |
| Rwork | 0.194 |
| R-free | 0.24953 |
| Structure solution method | MR |
| Starting model (for MR) | 3vdg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.081 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 107442 | |
| <I/σ(I)> | 7.4 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 0.1M TRIS-HCL, 0.8 M Potassium sodium tartrate tetrahydrate, 0.5% PEG MME5000 , pH 8.5, VAPOR DIFFUSION, HANGING DROP |
